Anticorps Polyclonal de lapin anti-CD31

CD31 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 33075-1-AP

Synonymes

Pecam, Pecam1, PECAM-1, Platelet endothelial cell adhesion molecule

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Galerie de données de validation

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  WB analysis using 33075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33075-1-AP (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat lung using 33075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 33075-1-AP (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 33075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 33075-1-AP (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 33075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 33075-1-AP (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 33075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 33075-1-AP (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules bEnd.3, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu pulmonaire de rat, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

33075-1-AP cible CD31 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD31 Protéine recombinante Eg1456
• Nom complet: platelet/endothelial cell adhesion molecule 1
• Masse moléculaire calculée: 76kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: NP_113779.1
• Symbole du gène: Pecam1
• Identification du gène (NCBI): 29583
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
rat	IHC	Int J Stem Cells; Collagen Scaffold Augments the Therapeutic Effect of Human Umbilical Cord Mesenchymal Stem Cells in a Rat Model of Intrauterine Adhesion; Authors - Linzhi Gao

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