Anticorps Recombinant de lapin anti-CD31

CD31 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242041E12

N° de cat : 84607-4-RR

Synonymes

242041E12, Pecam, PECAM 1, Pecam1, PECAM-1

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Galerie de données de validation

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  WB analysis using 84607-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84607-4-RR (CD31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of bEnd.3 using 84607-4-RR

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31/PECAM-1 antibody (84607-4-RR, Clone: 242041E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84607-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84607-4-RR against Mouse CD31 were performed. The affinity constant is 0.882 nM.

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Applications testées

• Résultats positifs en WB: cellules bEnd.3, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu splénique de souris, tissu cérébral de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules bEnd.3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84607-4-RR cible CD31 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD31 Protéine recombinante Eg0846
• Nom complet: platelet/endothelial cell adhesion molecule 1
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 100-130 kDa
• Numéro d’acquisition GenBank: NM_001305157.1
• Symbole du gène: CD31
• Identification du gène (NCBI): 18613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

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