Anticorps Recombinant de lapin anti-CD31

CD31 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242041B5

N° de cat : 98258-1-RR

Synonymes

242041B5, Pecam, PECAM 1, Pecam1, PECAM-1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98258-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD31 Rabbit RecAb (98258-1-RR, Clone: 242041B5) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98258-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD31 Rabbit RecAb (98258-1-RR, Clone: 242041B5) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98258-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98258-1-RR against Mouse CD31 were performed. The affinity constant is 0.547 nM.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98258-1-RR cible CD31 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD31 Protéine recombinante Eg0846
• Nom complet: platelet/endothelial cell adhesion molecule 1
• Masse moléculaire calculée: 81 kDa
• Numéro d’acquisition GenBank: NM_001305157.1
• Symbole du gène: CD31
• Identification du gène (NCBI): 18613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

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