Anticorps Recombinant de lapin anti-CD31

CD31 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

Biotin

CloneNo.

WM-59

N° de cat : Biotin-65614

Synonymes

EndoCAM, GPIIA, GPIIA', PECA1, PECAM 1

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Galerie de données de validation

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  FC experiment of human PBMCs using Biotin-65614

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD31 (WM-59) Mouse IgG2a RecAb (Biotin-65614, Clone: WM-59) and CoraLite®488-conjugated streptavidin, or unstained. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65614

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD31 (WM-59) Mouse IgG2a RecAb (Biotin-65614, Clone: WM-59) (red) and CoraLite®488-conjugated streptavidin, or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-65614 cible CD31 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: platelet/endothelial cell adhesion molecule
• Masse moléculaire calculée: 83 kDa
• Numéro d’acquisition GenBank: BC022512
• Symbole du gène: CD31
• Identification du gène (NCBI): 5175
• Conjugaison: Biotin
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

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