Anticorps Polyclonal de lapin anti-CD31

• Phare
• Validé par KD/KO

CD31 Polyclonal Antibody for IF/ICC, IF-P

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(2)

N° de cat : CL488-11265

Synonymes

PECAM1, PECAM 1, PECA1, GPIIA', GPIIA

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Galerie de données de validation

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  IF Staining of human tonsillitis using CL488-11265

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite® Plus 488 CD31 antibody (CL488-11265) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using CL488-11265

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD31 antibody (CL488-11265) at dilution of 1:200.

• 

  IF Staining of HUVEC using CL488-11265

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 CD31 antibody (CL488-11265) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF-P: tissu d'amygdalite humain,
• Résultats positifs en IF/ICC: cellules HUVEC,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

CL488-11265 cible CD31 dans les applications de IF/ICC, IF-P et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD31 Protéine recombinante Ag1787
• Nom complet: platelet/endothelial cell adhesion molecule
• Masse moléculaire calculée: 83 kDa
• Numéro d’acquisition GenBank: BC022512
• Symbole du gène: CD31
• Identification du gène (NCBI): 5175
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
human	IF	Biochem Biophys Res Commun; Dulaglutide restores endothelial progenitor cell levels in diabetic mice and mitigates high glucose-induced endothelial injury through SIRT1-mediated mitochondrial fission; Authors - Xi Mei
human	IF	FASEB J; CD74+ fibroblasts proliferate upon mechanical stretching to promote angiogenesis in keloids; Authors - Jingheng Zhang

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