Anticorps Monoclonal anti-CD31

CD31 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF/ICC, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

390

Avis (1)

N° de cat : CL488-65058

Synonymes

390, Pecam, PECAM 1, Pecam1, PECAM-1

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Galerie de données de validation

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  IF Staining of bEnd.3 using CL488-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite® Plus 488 CD31 antibody (CL488-65058, Clone: 390 ) at dilution of 1:100.

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  FC experiment of mouse bone marrow cells using CL488-65058

  1x10^6 mouse bone marrow cells were surface stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2), and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD31 (390) (CL488-65058, Clone: 390) or 0.25 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3). Cells were not fixed.

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  FC experiment of mouse bone marrow cells using CL488-65058

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD31 (390) (CL488-65058, Clone:390) (red) or 0.25 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: cellules bEnd.3,
• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65058 cible CD31 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de cellules progénitrices hématopoïétiques 32D de souris C3H/HeJ
• Nom complet: platelet/endothelial cell adhesion molecule 1
• Numéro d’acquisition GenBank: BC008519
• Symbole du gène: CD31
• Identification du gène (NCBI): 18613
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vanessa (Verified Customer) (09-27-2023)

Label but not very bright. Hard to distinguish from background.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Brain

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