Anticorps Monoclonal anti-CD31

CD31 Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF-P

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(1)

CloneNo.

3F8E2

N° de cat : CL594-66065

Synonymes

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

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Galerie de données de validation

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  IF Staining of human tonsillitis using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 2A1E2 ) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 2A1E2 ) at dilution of 1:200.

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  IF Staining of human placenta using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 3F8E2 ) at dilution of 1:200.

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  IF Staining of human placenta using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 3F8E2 ) at dilution of 1:200.

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  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in teal) and Phalloidin (in orange). DAPI in gray. Image credit: Shuntaro Yamada, PhD,

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  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in blue) and Phalloidin (in green). DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

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  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in purple) and Phalloidin (in teal). DAPI in yellow. Image credit: Shuntaro Yamada, PhD,

• 

  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in yellow) and Phalloidin (in green). DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

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Applications testées

• Résultats positifs en IF-P: tissu d'amygdalite humain, tissu placentaire humain

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

CL594-66065 cible CD31 dans les applications de WB, IF-P et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD31 Protéine recombinante Ag1787
• Nom complet: platelet/endothelial cell adhesion molecule
• Masse moléculaire calculée: 83 kDa
• Numéro d’acquisition GenBank: BC022512
• Symbole du gène: CD31
• Identification du gène (NCBI): 5175
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publications

Species	Application	Title
human	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang

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