Anticorps Recombinant de lapin anti-CD31

CD31 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

250372F8

N° de cat : 85898-4-RR

Synonymes

Pecam, Pecam1, PECAM-1, Platelet endothelial cell adhesion molecule

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Galerie de données de validation

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  WB analysis using 85898-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 85898-4-RR

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 85898-4-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 85898-4-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 85898-4-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 85898-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 85898-4-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 85898-4-RR (CD31 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat liver using 85898-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD31 antibody (85898-4-RR, Clone: 250372F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu pulmonaire de rat, tissu splénique de rat
• Résultats positifs en IHC: tissu hépatique de rat, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85898-4-RR cible CD31 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD31 Protéine recombinante Eg1456
• Nom complet: platelet/endothelial cell adhesion molecule 1
• Masse moléculaire calculée: 76kDa
• Poids moléculaire observé: 100-130 kDa
• Numéro d’acquisition GenBank: NP_113779.1
• Symbole du gène: Pecam1
• Identification du gène (NCBI): 29583
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

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