Anticorps Monoclonal anti-CD314/NKG2D

CD314/NKG2D Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

1D11

N° de cat : 65188-1-Ig

Synonymes

CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D

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Galerie de données de validation

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  FC experiment of human PBMCs using 65188-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD314/NKG2D (65188-1-Ig, Clone:1D11) or Mouse IgG1 Isotype Control and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000. Cells were then stained with CL488-conjugated CD8 (CL488-65135). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65188-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD314/NKG2D (65188-1-Ig, Clone:1D11) or Mouse IgG1 Isotype Control and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65188-1-Ig cible CD314/NKG2D dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée cellulaire NKL
• Nom complet: killer cell lectin-like receptor subfamily K, member 1
• Masse moléculaire calculée: 25 kDa
• Numéro d’acquisition GenBank: BC039836
• Symbole du gène: KLRK1
• Identification du gène (NCBI): 22914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par chromatographie d'affinité par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).

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