Anticorps Monoclonal anti-CD314/NKG2D

CD314/NKG2D Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Fluorescent Dye

CloneNo.

1D11

N° de cat : FITC-65188

Synonymes

CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-65188

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD8 and FITC Plus Anti-Human CD314/NKG2D (FITC-65188, Clone:1D11) or FITC Plus Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65188

  1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD314/NKG2D (FITC-65188, Clone:1D11) or FITC Plus Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65188 cible CD314/NKG2D dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée cellulaire NKL
• Nom complet: killer cell lectin-like receptor subfamily K, member 1
• Masse moléculaire calculée: 25 kDa
• Numéro d’acquisition GenBank: BC039836
• Symbole du gène: KLRK1
• Identification du gène (NCBI): 22914
• Conjugaison: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à l'isothiocyanate de fluorescéine (FITC) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).

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