Anticorps Monoclonal anti-FCGR2A / CD32a

FCGR2A / CD32a Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D3F1

N° de cat : 66529-1-Ig

Synonymes

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

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Galerie de données de validation

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  WB analysis using 66529-1-Ig

  Untreated and PNGase F-treated lysates of THP-1 cells and Mo7e cells were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 66529-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66529-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66529-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66529-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66529-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66529-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 66529-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCGR2A / CD32a antibody (66529-1-Ig, Clone: 1D3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules TF-1, cellules K-562, cellules Raji, cellules THP-1, cellules U-937
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66529-1-Ig cible FCGR2A / CD32a dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: FCGR2A / CD32a Protéine recombinante Ag25878
• Nom complet: Fc fragment of IgG, low affinity IIa, receptor (CD32)
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC020823
• Symbole du gène: CD32a
• Identification du gène (NCBI): 2212
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

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