Anticorps Polyclonal de lapin anti-CD320

• Phare
• Validé par KD/KO

CD320 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10343-1-PBS

Synonymes

8D6, 8D6A, FDC-signaling molecule 8D6, FDC-SM-8D6, TCblR

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Galerie de données de validation

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  WB analysis using 10343-1-AP (same clone as 10343-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10343-1-AP (CD320 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10343-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 10343-1-AP (same clone as 10343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10343-1-AP (CD320 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10343-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 10343-1-AP (same clone as 10343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10343-1-AP (CD320 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10343-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10343-1-PBS cible CD320 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD320 Protéine recombinante Ag0344
• Nom complet: CD320 molecule
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 60-70 kDa, 35-40 kDa
• Numéro d’acquisition GenBank: BC000668
• Symbole du gène: CD320
• Identification du gène (NCBI): 51293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa. This antibody raised against 84-280aa of human CD320 protein detects bands at 35-40 kDa and 60-70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

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