Anticorps Recombinant de lapin anti-CD320

CD320 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240500D4

N° de cat : 83574-3-RR

Synonymes

TCblR, FDC-SM-8D6, FDC-signaling molecule 8D6, 8D6A, 8D6

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Galerie de données de validation

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  WB analysis of HeLa using 83574-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83574-3-RR (CD320 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83574-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83574-3-RR against Human CD320 were performed. The affinity constant is 6.55 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules K-562, cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83574-3-RR cible CD320 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD320 Protéine recombinante Ag21445
• Nom complet: CD320 molecule
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC000668
• Symbole du gène: CD320
• Identification du gène (NCBI): 51293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa. This antibody raised against 84-280aa of human CD320 protein detects bands at 58 kDa and 60-70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

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