Anticorps Monoclonal anti-E-cadherin

E-cadherin Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

canin, Humain, souris

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

DECMA-1

N° de cat : PE-65241

Synonymes

CD324 (E-cadherin), cadherin 1, Cdh1, Ecad, L CAM

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Galerie de données de validation

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  FC experiment of MDCK using PE-65241

  1x10^6 MDCK cells were surface stained with 0.2 ug PE Anti-Mouse CD324 (E-cadherin) (DECMA-1) (PE-65241, Clone:DECMA-1), or 0.2 ug PE Rat IgG1 Isotype Control (HRPN) (PE-65212, Clone: HRPN). Cells were not fixed.

• 

  FC experiment of MDCK using PE-65241

  1x10^6 MDCK cells were surface stained with 0.2 ug PE Anti-Mouse CD324 (E-cadherin) (DECMA-1) (PE-65241, Clone:DECMA-1) (red), or 0.2 ug PE Rat IgG1 Isotype Control (HRPN) (PE-65212, Clone: HRPN) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules MDCK,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65241 cible E-cadherin dans les applications de FC et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Mouse embryonal carcinoma cell line PCC4 Aza RI
• Nom complet: cadherin 1
• Numéro d’acquisition GenBank: BC098501
• Symbole du gène: E-cadherin
• Identification du gène (NCBI): 12550
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: N/A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

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