Anticorps Polyclonal de lapin anti-CD34
CD34 Polyclonal Antibody for IF, IHC, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugaison
Non conjugué
N° de cat : 14486-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HL-60, cellules TF-1 |
| Résultats positifs en IHC | tissu d'amygdalite humain, tissu de cancer du foie humain, tissu de gliome humain, tissu d'hystéromyome humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | tissu d'amygdalite humain, tissu de cancer du foie humain, tissu placentaire humain |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochimie (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 15 publications below |
| IHC | See 37 publications below |
| IF | See 17 publications below |
| FC | See 2 publications below |
Informations sur le produit
14486-1-AP cible CD34 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | bovin, Humain, Lapin |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | CD34 Protéine recombinante Ag5887 |
| Nom complet | CD34 molecule |
| Masse moléculaire calculée | 41 kDa |
| Poids moléculaire observé | 100-120 kDa |
| Numéro d’acquisition GenBank | BC039146 |
| Symbole du gène | CD34 |
| Identification du gène (NCBI) | 947 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for CD34 antibody 14486-1-AP | Download protocol |
| IHC protocol for CD34 antibody 14486-1-AP | Download protocol |
| IF protocol for CD34 antibody 14486-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma | ||
Sci Adv Self-powered enzyme-linked microneedle patch for scar-prevention healing of diabetic wounds | ||
Nat Commun ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer. | ||
J Hepatol Intratumoral neutrophils: a poor prognostic factor for hepatocellular carcinoma following resection. | ||
Theranostics Ligand Activation of PPARγ by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1α. | ||
Bioact Mater Novel magnetic silk fibroin scaffolds with delayed degradation for potential long-distance vascular repair. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Madan Kumar (Verified Customer) (07-13-2021) | Rat primary hepatocyte cells were subjected to SDS PAGE and performed western blot analysis with CD34 antibody (Cat log# 14486-1-AP). This antibody works good.
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