Anticorps Monoclonal anti-CD34

CD34 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3C8G12

N° de cat : 60180-1-PBS

Synonymes

3C8G12, CD34 molecule

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Galerie de données de validation

• 

  WB analysis of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60180-1-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human uterus using 60180-1-Ig (same clone as 60180-1-PBS)

  human uterus tissue were subjected to SDS PAGE followed by western blot with 60180-1-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 60180-1-Ig (same clone as 60180-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60180-1-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60180-1-Ig (CD34 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60180-1-Ig (CD34 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, magenta), ASGR1 antibody (11739-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA502, orange), CoraLite® Plus 488 CD163 antibody (CL488-16646, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

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Applications publiées

• IF: See 1 publications below

Informations sur le produit

60180-1-PBS cible CD34 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD34 Protéine recombinante Ag5996
• Nom complet: CD34 molecule
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC039146
• Symbole du gène: CD34
• Identification du gène (NCBI): 947
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).

Publications

Species	Application	Title
human	IF	Acta Pharmacol Sin; Genetic and pharmacological targeting of Snail inhibits atherosclerosis by relieving intraplaque endothelium dysfunction and associated inflammation; Authors - Bo-Xue Ren

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