Anticorps Recombinant de lapin anti-CD34

CD34 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

242069F8

N° de cat : 84242-5-RR

Synonymes

242069F8, CD34 molecule

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Galerie de données de validation

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  WB analysis using 84242-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84242-5-RR (CD34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 84242-5-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 84242-5-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84242-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84242-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84242-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84242-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 84242-5-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 84242-5-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 84242-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD34 antibody (84242-5-RR, Clone: 242069F8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84242-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84242-5-RR against Human CD34 were performed. The affinity constant is 16.3 nM.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules bEnd.3, cellules HUVEC, tissu placentaire humain, tissu testiculaire de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu d'amygdalite humain, tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu placentaire humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84242-5-RR cible CD34 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD34 molecule
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC039146
• Symbole du gène: CD34
• Identification du gène (NCBI): 947
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

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