Anticorps Monoclonal anti-CD34

CD34 Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

IF-P

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3C8G12

N° de cat : CL594-60180

Synonymes

CD34, CD34 molecule

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Galerie de données de validation

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  IF Staining of human liver cancer using CL594-60180

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 CD34 antibody (CL594-60180, Clone: 3C8G12 ) at dilution of 1:100, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta), CoraLite® Plus 488 CD163 antibody (CL488-16646, green).

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  IF Staining of human placenta using CL594-60180

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite®594 CD34 antibody (CL594-60180, Clone: 3C8G12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using CL594-60180

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite®594 CD34 antibody (CL594-60180, Clone: 3C8G12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using CL594-60180

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-60180 (CD34 antibody), at dilution of 1:100.

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  IF Staining of human tonsillitis using CL594-60180

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-60180 (CD34 antibody), at dilution of 1:100.

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Applications testées

• Résultats positifs en IF-P: tissu de cancer du foie humain, tissu d'amygdalite humain, tissu placentaire humain

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60180 cible CD34 dans les applications de IF-P et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD34 Protéine recombinante Ag5996
• Nom complet: CD34 molecule
• Masse moléculaire calculée: 41 kDa
• Numéro d’acquisition GenBank: BC039146
• Symbole du gène: CD34
• Identification du gène (NCBI): 947
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

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