Anticorps Monoclonal anti-GITR/TNFRSF18

GITR/TNFRSF18 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

FITC Fluorescent Dye

Publications(1)

CloneNo.

DTA-1

N° de cat : FITC-65102

Synonymes

CD357, GITR, Tnfrsf18, AITR, Glucocorticoid-induced TNFR-related protein

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Galerie de données de validation

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  FC experiment of mouse splenocytes using FITC-65102

  1X10^6 mouse splenocytes were surface stained with 0.125 ug PE-Anti-Mouse CD4 (PE-65104, clone GK1.5), 0.125 ug APC-Anti-Mouse CD25 (APC-65137, clone PC61.5) and 0.06 ug FITC-Anti-Mouse CD357 (GITR) (FITC-65102, clone DTA-1). Cells were not fixed. CD4+ lymphocytes were gated for analysis of CD25 and CD357 (GITR) staining.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

FITC-65102 cible GITR/TNFRSF18 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes T CD25+ CD4+ de souris
• Nom complet: tumor necrosis factor receptor superfamily, member 18
• Numéro d’acquisition GenBank: BC146517
• Symbole du gène: CD357
• Identification du gène (NCBI): 21936
• Conjugaison: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.1% gelatin
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).

Publications

Species	Application	Title
mouse	IF	Adv Healthc Mater; Engineered Hollow Nanocomplex Combining Photothermal and Antioxidant Strategies for Targeted Tregs Depletion and Potent Immune Activation in Tumor Immunotherapy; Authors - Qi Sun

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