Anticorps Monoclonal anti-CD357 (GITR)

CD357 (GITR) Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

DTA-1

N° de cat : CL488-65102

Synonymes

AITR, CD357, CD357 (GITR), Gitr, Tnfrsf18

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL488-65102

  1X10^6 mouse splenocytes were surface stained with PE-Anti-Mouse CD4 (PE-65104, Clone: GK1.5), CoraLite® Plus 647-Conjugated Anti-Mouse CD25 (CL647-65137, Clone: PC61.5) and 5 ul CoraLite® Plus 488 Anti-Mouse CD357 (GITR) (CL488-65102, Clone: DTA-1). Cells were not fixed. CD4+ lymphocytes were gated for analysis of CD25 and CD357 (GITR) staining.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65102 cible CD357 (GITR) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes T CD25+ CD4+ de souris
• Nom complet: tumor necrosis factor receptor superfamily, member 18
• Numéro d’acquisition GenBank: BC146517
• Symbole du gène: Tnfrsf18
• Identification du gène (NCBI): 21936
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).