Anticorps Monoclonal anti-GITR/TNFRSF18

GITR/TNFRSF18 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

DTA-1

N° de cat : CL488-65102

Synonymes

CD357, GITR, Tnfrsf18, AITR, Glucocorticoid-induced TNFR-related protein

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL488-65102

  1X10^6 mouse splenocytes were surface stained with PE-Anti-Mouse CD4 (PE-65104, Clone: GK1.5), CoraLite® Plus 647-Conjugated Anti-Mouse CD25 (CL647-65137, Clone: PC61.5) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD357 (GITR) (CL488-65102, Clone: DTA-1). Cells were not fixed. CD4+ lymphocytes were gated for analysis of CD25 and CD357 (GITR) staining.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65102 cible GITR/TNFRSF18 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes T CD25+ CD4+ de souris
• Nom complet: tumor necrosis factor receptor superfamily, member 18
• Numéro d’acquisition GenBank: BC146517
• Symbole du gène: CD357
• Identification du gène (NCBI): 21936
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).

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