Anticorps Recombinant de lapin anti-CD37

CD37 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250262A3

N° de cat : 85917-1-PBS

Synonymes

Leukocyte antigen CD37, Tetraspanin 26, TSPAN26

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Galerie de données de validation

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  WB analysis using 85917-1-RR (same clone as 85917-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85917-1-RR (Cd37 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85917-1-RR (same clone as 85917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85917-1-RR (CD37 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85917-1-RR (same clone as 85917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85917-1-RR (CD37 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 85917-1-RR (same clone as 85917-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD37 antibody (85917-1-RR, Clone: 250262A3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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  IF Staining of rat spleen using 85917-1-RR (same clone as 85917-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD37 antibody (85917-1-RR, Clone: 250262A3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A20 using 85917-1-RR (same clone as 85917-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A20 cells using Cd37 antibody (85917-1-RR, Clone: 250262A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85917-1-PBS cible CD37 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD37 Protéine recombinante Eg2729
• Nom complet: CD37 antigen
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_007645.4
• Symbole du gène: Cd37
• Identification du gène (NCBI): 12493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).

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