Anticorps Polyclonal de lapin anti-CD38

CD38 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 25284-1-AP

Synonymes

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

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Galerie de données de validation

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  WB analysis using 25284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25284-1-AP (CD38 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 25284-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25284-1-AP (CD38 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 25284-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 25284-1-AP (CD38 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 25284-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 25284-1-AP (CD38 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HL-60 using 25284-1-AP

  1X10^6 HL-60 cells were stained with 0.2ug CD38 antibody (25284-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Daudi, cellules HL-60, cellules Ramos
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HL-60

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

25284-1-AP cible CD38 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD38 Protéine recombinante Ag17868
• Nom complet: CD38 molecule
• Masse moléculaire calculée: 300 aa, 34 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC007964
• Symbole du gène: CD38
• Identification du gène (NCBI): 952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Publications

Species	Application	Title
human	IHC	Front Oncol; CD38 Multi-Functionality in Oral Squamous Cell Carcinoma: Prognostic Implications, Immune Balance, and Immune Checkpoint.; Authors - Zhuang Ding
human	IF	Cell Commun Signal; TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma.; Authors - Hua Bai
human	WB,IHC	Oncol Rep; CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer.; Authors - Shan Liao
human	WB	PLoS Pathog; The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness; Authors - Elena Sugrue