Anticorps Monoclonal anti-CD38

• Phare
• Validé par KD/KO

CD38 Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF/ICC, IF-P, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(19)

CloneNo.

3C6G4

Avis (1)

N° de cat : 60006-1-Ig

Synonymes

2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, 3C6G4, ADP ribosyl cyclase 1

---

Galerie de données de validation

• 

  WB analysis using 60006-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60006-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse spleen using 60006-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 60006-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 60006-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  Affinity and Kinetic Characterization of 60006-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60006-1-Ig against Human CD38 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules Daudi, cellules Jurkat, cellules Raji, cellules Ramos, cellules TF-1, cellules THP-1
• Résultats positifs en IF-P: tissu splénique de souris,
• Résultats positifs en IF/ICC: cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IF: See 6 publications below

Informations sur le produit

60006-1-Ig cible CD38 dans les applications de WB, IF/ICC, IF-P, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD38 Protéine recombinante Ag0487
• Nom complet: CD38 molecule
• Masse moléculaire calculée: 300 aa, 34 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC007964
• Symbole du gène: CD38
• Identification du gène (NCBI): 952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Publications

Species	Application	Title
human	WB	Nat Immunol; PD-1 blockade in subprimed CD8 cells induces dysfunctional PD-1+CD38hi cells and anti-PD-1 resistance.; Authors - Vivek Verma ; KD Validated
human	WB	Pharmacol Res; Targeting CD38/ ADP-ribosyl cyclase as a novel therapeutic strategy for identification of three potent agonists for leukopenia treatment; Authors - Yuanzhi Liu
mouse	WB	J Agric Food Chem; Pelargonidin-3-O-galactoside Alleviates Acute Lung Injury Induced by Klebsiella pneumoniae by Targeting CD38-Mediated Pyroptosis; Authors - Fei Jiang
mouse	WB	Glia; Activation of astrocytic sigma-1 receptor exerts antidepressant-like effect via facilitating CD38-driven mitochondria transfer.; Authors - Yun Wang ; KD Validated
human		Cancer Cell Int; Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.; Authors - Runzhi Huang
	IF	Cell Rep; Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.; Authors - Huiwen Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Komuraiah (Verified Customer) (07-16-2021)

CD38 expression that I tested in kidney tissue from control and cisplatin induced kidney injury model. This antibody is working great with high specificity with suggested molecular size.

• Applications: Western Blot

CD38 Antibody Western Blot validation ( dilution) in (Cat no:60006-1-Ig)

{{ptg:RelatedPrimaryAntibodies}}