Anticorps Monoclonal anti-CD38

• Phare
• Validé par KD/KO

CD38 Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

3C6G4

N° de cat : 60006-1-PBS

Synonymes

2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, 3C6G4, ADP ribosyl cyclase 1

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Galerie de données de validation

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  WB analysis using 60006-1-Ig (same clone as 60006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

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  WB analysis using 60006-1-Ig (same clone as 60006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 60006-1-Ig (same clone as 60006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 60006-1-Ig (same clone as 60006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 60006-1-Ig (same clone as 60006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 60006-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60006-1-Ig against Human CD38 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

60006-1-PBS cible CD38 dans les applications de WB, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD38 Protéine recombinante Ag0487
• Nom complet: CD38 molecule
• Masse moléculaire calculée: 300 aa, 34 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC007964
• Symbole du gène: CD38
• Identification du gène (NCBI): 952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

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