Anticorps Recombinant de lapin anti-CD38

CD38 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241246D5

N° de cat : 84070-6-PBS

Synonymes

ADPRC 1, ADP ribosyl cyclase 1, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase

---

Galerie de données de validation

• 

  WB analysis using 84070-6-RR (same clone as 84070-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84070-6-RR (CD38 antibody) at dilution of 1:17000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84070-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84070-6-RR (same clone as 84070-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-6-RR (CD38 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84070-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84070-6-RR (same clone as 84070-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-6-RR (CD38 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84070-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84070-6-RR (same clone as 84070-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-6-RR (CD38 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84070-6-PBS in a different storage buffer formulation.

• 

  IF Staining of Raji using 84070-6-RR (same clone as 84070-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (84070-6-RR, Clone: 241246D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84070-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84070-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84070-6-RR against Human CD38 were performed. The affinity constant is 1.11 nM.

---

Informations sur le produit

84070-6-PBS cible CD38 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD38 molecule
• Masse moléculaire calculée: 300 aa, 34 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC007964
• Symbole du gène: CD38
• Identification du gène (NCBI): 952
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

{{ptg:RelatedPrimaryAntibodies}}