Anticorps Monoclonal anti-CD3

• Phare
• Validé par KD/KO

CD3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

31

CloneNo.

3F3A1

N° de cat : 60181-1-Ig

Synonymes

CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 60181-1-Ig

  WB result of CD3 antibody (60181-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells.

• 

  WB analysis using 60181-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60181-1-Ig (CD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60181-1-Ig(CD3E antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 60181-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green).

• 

  IF Staining of human tonsillitis using 60181-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green).

• 

  IF Staining of human tonsillitis using 60181-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60181-1-Ig (CD3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of Jurkat using 60181-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60181-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (60181-1-Ig, Clone: 3F3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD20 antibody (24828-1-AP, green).

• 

  FC experiment of Jurkat using 60181-1-Ig

  1X10^6 Jurkat cells were stained with 0.20ug CD3 antibody (60181-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules MOLT-4, cellules Jurkat, CMSP humaines
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain, cellules Jurkat
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:3200-1:12800
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 17 publications below
• IF: See 13 publications below
• FC: See 1 publications below

Informations sur le produit

60181-1-Ig cible CD3 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD3 Protéine recombinante Ag11797
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3E
• Identification du gène (NCBI): 916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.

Publications

Species	Application	Title
human	IF	J Med Virol; Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy; Authors - Xinyue Deng
human	IF	EBioMedicine; Patient-derived melanoma organoid models facilitate the assessment of immunotherapies; Authors - Lingling Ou
	IHC	Autophagy; Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.; Authors - Jingru Bai
	IHC	Carbohydr Polym; Multifunctional chitin-based hollow nerve conduit for peripheral nerve regeneration and neuroma inhibition.; Authors - Xiaqing Yang
	IHC	Pharmacol Res; CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.; Authors - Guopin Pan
human	IHC	Cancer Immunol Immunother; Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.; Authors - Pei Yang

Produits associés

CD3 Polyclonal antibody

17617-1-AP

CD3 Gamma Polyclonal antibody

21120-1-AP

CD3 Delta Polyclonal antibody

16669-1-AP

CD247 Polyclonal antibody

12837-2-AP

CD43 Monoclonal antibody

66224-1-Ig

CD7 Monoclonal antibody

60209-2-Ig

CD4 Polyclonal antibody

11056-2-AP

CD8 Polyclonal antibody

21256-1-AP