Anticorps Polyclonal de lapin anti-CD3EAP

CD3EAP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31162-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 31162-1-AP (same clone as 31162-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31162-1-AP (CD3EAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31162-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 31162-1-AP (same clone as 31162-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD3EAP antibody (31162-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31162-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31162-1-PBS cible CD3EAP dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD3EAP Protéine recombinante Ag35074
• Nom complet: CD3e molecule, epsilon associated protein
• Masse moléculaire calculée: 510 aa, 55 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC038992
• Symbole du gène: CD3EAP
• Identification du gène (NCBI): 10849
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.

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