Anticorps Recombinant de lapin anti-CD3EAP

CD3EAP Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241379H5

N° de cat : 84139-4-RR

Synonymes

241379H5, A34.5, Antisense to ERCC-1 protein, ASE1, ASE-1

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Galerie de données de validation

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  WB analysis using 84139-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84139-4-RR (CD3EAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 84139-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CD3EAP antibody (84139-4-RR, Clone: 241379H5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 84139-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD3EAP antibody (84139-4-RR, Clone: 241379H5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U2OS using 84139-4-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug CD3EAP Recombinant antibody (84139-4-RR, Clone:241379H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84139-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84139-4-RR against Human CD3EAP were performed. The affinity constant is 0.339 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HCT 116, cellules HEK-293T, cellules HeLa, cellules K-562, cellules NCI-H1299
• Résultats positifs en IF/ICC: cellules A431, cellules A549
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84139-4-RR cible CD3EAP dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD3EAP Protéine recombinante Ag35074
• Nom complet: CD3e molecule, epsilon associated protein
• Masse moléculaire calculée: 510 aa, 55 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC038992
• Symbole du gène: CD3EAP
• Identification du gène (NCBI): 10849
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.

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