Anticorps Polyclonal de lapin anti-CD4

CD4 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, FC, ELISA

Conjugaison

Non conjugué

Publications(19)

Avis (1)

N° de cat : 11056-2-AP

Synonymes

CD4, CD4 molecule, CD4mut

---

Galerie de données de validation

• 

  WB analysis of human spleen using 11056-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11056-2-AP (CD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen tissue using 11056-2-AP

  IP result of anti-CD4 (11056-2-AP for IP and Detection) with mouse spleen tissue lysate.

---

Applications testées

• Résultats positifs en WB: tissu splénique humain
• Résultats positifs en IP: tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Informations sur le produit

11056-2-AP cible CD4 dans les applications de WB, IP, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD4 Protéine recombinante Ag0422
• Nom complet: CD4 molecule
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC025782
• Symbole du gène: CD4
• Identification du gène (NCBI): 920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).

Publications

Species	Application	Title
mouse		ACS Appl Mater Interfaces; NIR-Triggered Sequentially Responsive Nanocarriers Amplified Cascade Synergistic Effect of Chemo-Photodynamic Therapy with Inspired Antitumor Immunity.; Authors - Feiyang Jin
human		Cancer Lett; A PD-1 peptide antagonist exhibits potent anti-tumor and immune regulatory activity.; Authors - Huimin Tao
human	WB	Front Immunol; PCBP1 interacts with the HTLV-1 Tax oncoprotein to potentiate NF-κB activation; Authors - Rui Su
mouse		EBioMedicine; Enho Mutations Causing Low Adropin: A Possible Pathomechanism of MPO-ANCA Associated Lung Injury.; Authors - Feng Gao
		Am J Cancer Res; Changes in the intestinal microecology induced by bacillus subtilis inhibit the occurrence of ulcerative colitis and associated cancers: a study on the mechanisms.; Authors - Chuancong Wu
mouse		J Transl Med; Plasma proteomic analysis of autoimmune hepatitis in an improved AIH mouse model.; Authors - Han Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Luca (Verified Customer) (02-26-2018)

• Applications: Other (please state below),
• Primary Antibody Dilution: 10000 to 1 ng/ml

{{ptg:RelatedPrimaryAntibodies}}