Anticorps Polyclonal de lapin anti-CD4

CD4 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 19068-1-AP

Synonymes

CD4, CD4 molecule, CD4mut

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Galerie de données de validation

• 

  WB analysis using 19068-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19068-1-AP (CD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig thymus using 19068-1-AP

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 19068-1-AP (CD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19068-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19068-1-AP (CD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse thymus using 19068-1-AP

  IP Result of anti-CD4 (IP:19068-1-AP, 4ug; Detection:19068-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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Applications testées

• Résultats positifs en WB: cellules U-937, tissu de thymus de porc, tissu de thymus de souris
• Résultats positifs en IP: tissu de thymus de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

19068-1-AP cible CD4 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD4 Protéine recombinante Ag13616
• Nom complet: CD4 molecule
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC025782
• Symbole du gène: CD4
• Identification du gène (NCBI): 920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).

Publications

Species	Application	Title
mouse		ACS Appl Mater Interfaces; Fe(III)-Chelated Polydopamine Nanoparticles for Synergistic Tumor Therapies of Enhanced Photothermal Ablation and Antitumor Immune Activation.; Authors - Na Xu
mouse	WB	J Control Release; Cancer-cell-biomimetic Upconversion nanoparticles combining chemo-photodynamic therapy and CD73 blockade for metastatic triple-negative breast cancer.; Authors - Feiyang Jin
human		J Hematol Oncol; PD-1 axis expression in musculoskeletal tumors and antitumor effect of nivolumab in osteosarcoma model of humanized mouse.; Authors - Bingxin Zheng
rat		Int J Mol Sci; Human Milk Oligosaccharide 2'-Fucosyllactose Induces Neuroprotection from Intracerebral Hemorrhage Stroke.; Authors - Tsai-Wei Hung
rat		Exp Neurol; Calpain mediated expansion of CD4+ cytotoxic T cells in rodent models of Parkinson's disease.; Authors - Azizul Haque
mouse		Food Funct; Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway.; Authors - Jinhong Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nicole (Verified Customer) (04-28-2022)

no signal expression is controlled by other antibodies

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela, Plasmid driven expression of various CD4 fusion constructs

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