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Anticorps Monoclonal anti-CD4

CD4 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

OKT4

N° de cat : 65134-1-Ig

Synonymes

CD4, CD4 molecule, CD4mut

Galerie de données de validation

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (OKT4) (65134-1-Ig)

    FC experiment of human PBMCs using 65134-1-Ig

    1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD3 and 0.2 ug Anti-Human CD4 (65134-1-Ig, Clone:OKT4) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (OKT4) (65134-1-Ig)

    FC experiment of human PBMCs using 65134-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65134-1-Ig, Clone:OKT4) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

Applications testées

Résultats positifs en cytométrieCMSP humaines,

Dilution recommandée

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65134-1-Ig cible CD4 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2b, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Lymphocytes T périphériques humains
Nom complet CD4 molecule
Masse moléculaire calculée 55 kDa
Numéro d’acquisition GenBankBC025782
Symbole du gène CD4
Identification du gène (NCBI) 920
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide.
Conditions de stockageStore at 2-8°C. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocole

Product Specific Protocols
FC protocol for CD4 antibody 65134-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sunil (Verified Customer) (08-29-2023)

Use to conjugate our own synthesised dyes in our own lab and tested with ultracomp beads and cytotrol cells.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 5ul used per test
  • Cell Tissue Type: ultracomp beads