Anticorps Monoclonal anti-CD4

CD4 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF, FC

Conjugaison

Non conjugué

Publications(1)

CloneNo.

RPA-T4

Avis (3)

N° de cat : 65143-1-Ig

Synonymes

CD4 molecule, CD4mut, RPA-T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Galerie de données de validation

• 

  FC experiment of human PBMCs using 65143-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) or Mouse IgG1 Isotype Control and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD3 (CL488-65151). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65143-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) or Mouse IgG1 Isotype Control and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

65143-1-Ig cible CD4 dans les applications de IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD4 molecule
• Masse moléculaire calculée: 55 kDa
• Numéro d’acquisition GenBank: BC025782
• Symbole du gène: CD4
• Identification du gène (NCBI): 920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

Species	Application	Title
human	IF	Allergy Asthma Immunol Res; TET2 Regulates 5-Hydroxymethylcytosine Signature and CD4+ T-Cell Balance in Allergic Rhinitis.; Authors - Lu Tan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sunil (Verified Customer) (08-03-2023)

worked as expected for conjugating our custom dyes to

• Applications: Flow Cytometry

FH

Sareena (Verified Customer) (06-02-2023)

Worked well with compensation beads

• Applications: Immunofluorescence, Flow Cytometry

FH

Sareena (Verified Customer) (04-20-2023)

Worked well with conjugating fluorophores and tested with compensation beads for flow cytometry analysis.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 100 ug
• Cell Tissue Type: CD4 RPA-T4 antibody

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