Anticorps Recombinant de lapin anti-CD4

CD4 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

OKT4

N° de cat : 65505-1-PBS

Synonymes

CD4 molecule, CD4mut, OKT4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Galerie de données de validation

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  FC experiment of human PBMCs using 65505-1-PBS

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65505-1-RR (same clone as 65505-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-RR, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65505-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65505-1-RR (same clone as 65505-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-RR, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65505-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65505-1-PBS

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65505-1-PBS

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.1 ug Anti-Human CD4 Mouse Monoclonal Antibody (Clone:OKT4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65505-1-RR (same clone as 65505-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-RR, Clone:OKT4) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.1 ug Anti-Human CD4 Mouse Monoclonal Antibody (Clone:OKT4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65505-1-PBS in a different storage buffer formulation.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65505-1-PBS cible CD4 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Lymphocytes T périphériques humains
• Nom complet: CD4 molecule
• Masse moléculaire calculée: 55 kDa
• Numéro d’acquisition GenBank: BC025782
• Symbole du gène: CD4
• Identification du gène (NCBI): 920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

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