Anticorps Recombinant de lapin anti-CD4

CD4 Recombinant Antibody for FC

Hôte / Isotype

Rat / IgG2a

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

GK1.5

N° de cat : 65662-1-PBS

Synonymes

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 65662-1-TR (same clone as 65662-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD4 (GK1.5) Rat IgG2a RecAb (65662-1-TR, Clone: GK1.5) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone: 145-2C11). Cells were not fixed. This data was developed using the same antibody clone with 65662-1-PBS in a different storage buffer formulation.

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  FC experiment of mouse splenocytes using 65662-1-TR (same clone as 65662-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD4 (GK1.5) Rat IgG2a RecAb (65662-1-TR, Clone: GK1.5) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed. This data was developed using the same antibody clone with 65662-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65662-1-PBS cible CD4 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD4 antigen
• Numéro d’acquisition GenBank: BC039137
• Symbole du gène: Cd4
• Identification du gène (NCBI): 12504
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

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