Anticorps Recombinant de lapin anti-CD4

CD4 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, primates non humains, singe

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240427E12

N° de cat : 98042-1-PBS

Synonymes

240427E12, CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Galerie de données de validation

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  FC experiment of human PBMCs using 98042-1-RR (same clone as 98042-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone: 240427E12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98042-1-PBS in a different storage buffer formulation.

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  FC experiment of Cnyo PBMCs using 98042-1-RR (same clone as 98042-1-PBS)

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone:240427E12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98042-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Cnyo PBMCs using 98042-1-RR (same clone as 98042-1-PBS)

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone:240427E12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98042-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98042-1-RR (same clone as 98042-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone: 240427E12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98042-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98042-1-RR against Human CD4 were performed. The affinity constant is 3.88 nM.

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Informations sur le produit

98042-1-PBS cible CD4 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains, singe

• Réactivité: Humain, primates non humains, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD4 Protéine recombinante Eg0333
• Nom complet: CD4 molecule
• Masse moléculaire calculée: 55 kDa
• Numéro d’acquisition GenBank: BC025782
• Symbole du gène: CD4
• Identification du gène (NCBI): 920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

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