Anticorps Recombinant de lapin anti-CD4

CD4 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, primates non humains, singe

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240427E12

N° de cat : 98042-1-RR

Synonymes

240427E12, CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Galerie de données de validation

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  FC experiment of human PBMCs using 98042-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone: 240427E12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of Cnyo PBMCs using 98042-1-RR

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone:240427E12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  FC experiment of Cnyo PBMCs using 98042-1-RR

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone:240427E12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98042-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone: 240427E12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

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  Affinity and Kinetic Characterization of 98042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98042-1-RR against Human CD4 were performed. The affinity constant is 3.88 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines, cynomolgus PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98042-1-RR cible CD4 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains, singe

• Réactivité: Humain, primates non humains, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD4 Protéine recombinante Eg0333
• Nom complet: CD4 molecule
• Masse moléculaire calculée: 55 kDa
• Numéro d’acquisition GenBank: BC025782
• Symbole du gène: CD4
• Identification du gène (NCBI): 920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

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