Anticorps Recombinant de lapin anti-CD4

CD4 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250263B7

N° de cat : 98522-3-PBS

Synonymes

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98522-3-RR (same clone as 98522-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse CD4 Rabbit RecAb (98522-3-RR, Clone: 250263B7) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98522-3-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98522-3-RR (same clone as 98522-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse CD4 Rabbit RecAb (98522-3-RR, Clone: 250263B7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98522-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98522-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98522-3-RR against Mouse CD4 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

98522-3-PBS cible CD4 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD4 Protéine recombinante Eg3847
• Nom complet: CD4 antigen
• Masse moléculaire calculée: 51 kDa
• Numéro d’acquisition GenBank: NM_013488
• Symbole du gène: Cd4
• Identification du gène (NCBI): 12504
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

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