Anticorps Recombinant de lapin anti-CD4

CD4 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250263B7

N° de cat : 98522-3-RR

Synonymes

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98522-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse CD4 Rabbit RecAb (98522-3-RR, Clone: 250263B7) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of mouse splenocytes using 98522-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.125 ug Anti-Mouse CD4 Rabbit RecAb (98522-3-RR, Clone: 250263B7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98522-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98522-3-RR against Mouse CD4 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98522-3-RR cible CD4 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD4 Protéine recombinante Eg3847
• Nom complet: CD4 antigen
• Masse moléculaire calculée: 51 kDa
• Numéro d’acquisition GenBank: NM_013488
• Symbole du gène: Cd4
• Identification du gène (NCBI): 12504
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

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