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Anticorps Monoclonal anti-CD4

CD4 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

SK3

N° de cat : APC-65147

Synonymes

CD4, CD4 molecule, CD4mut, SK3

Galerie de données de validation

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using APC Anti-Human CD4 (SK3) (APC-65147)

    FC experiment of human peripheral blood lymphocytes using APC-65147

    1X10^6 human peripheral blood lymphocytes were surface stained with FITC Anti-Human CD3 (FITC-65151, clone UCHT1) and 5.00 ul/test APC Anti-Human CD4 (APC-65147, Clone:SK3). Sample were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using APC Anti-Human CD4 (SK3) (APC-65147)

    FC experiment of human peripheral blood lymphocytes using APC-65147

    1X10^6 human peripheral blood lymphocytes were surface stained with FITC Anti-Human CD3 (FITC-65151, clone UCHT1) and APC mouse IgG1 isotype control antibody. Sample were not fixed.

Applications testées

Résultats positifs en cytométrielymphocytes de sang périphérique humain

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65147 cible CD4 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet CD4 molecule
Masse moléculaire calculée 55 kDa
Numéro d’acquisition GenBankBC025782
Symbole du gène CD4
Identification du gène (NCBI) 920
Conjugaison APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocole

Product Specific Protocols
FC protocol for APC CD4 antibody APC-65147Download protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Macarena Lucia (Verified Customer) (09-28-2022)

Good staining at recommended dilution

  • Applications: Flow Cytometry
  • Cell Tissue Type: primary T cell