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Anticorps Recombinant de lapin anti-CD40

CD40 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240874B2

N° de cat : 83972-5-PBS

Synonymes

Tnfrsf5, CD40 antigen, Bp50, 240874B2

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using CD40 Recombinant antibody (83972-5-RR)

    WB analysis using 83972-5-RR (same clone as 83972-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83972-5-RR (CD40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83972-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83972-5-RR

    Affinity and Kinetic Characterization of 83972-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83972-5-RR against Mouse CD40 were performed. The affinity constant is 4.50 nM.

Informations sur le produit

83972-5-PBS cible CD40 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Protéine recombinante
Nom complet CD40 antigen
Masse moléculaire calculée32 kDa
Poids moléculaire observé40-45 kDa
Numéro d’acquisition GenBankNM_011611.2
Symbole du gène Cd40
Identification du gène (NCBI) 21939
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.



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