Anticorps Recombinant de lapin anti-CD40

CD40 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

241760A9

N° de cat : APC-98191

Synonymes

241760A9, B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor

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Galerie de données de validation

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  FC experiment of human PBMCs using APC-98191

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD19, and 5 ul APC Anti-Human CD40 Rabbit RecAb (APC-98191, Clone: 241760A9) or APC Rabbit IgG Isotype Control RecAb (APC-98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using APC-98191

  1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD40 Rabbit RecAb (APC-98191, Clone: 241760A9) (red) or APC Rabbit IgG Isotype Control RecAb (APC-98136, Clone: 240953C9) (blue). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-98191 cible CD40 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD40 Protéine recombinante Eg1797
• Nom complet: CD40 molecule, TNF receptor superfamily member 5
• Masse moléculaire calculée: 31kDa
• Numéro d’acquisition GenBank: NM_001250.6
• Symbole du gène: CD40
• Identification du gène (NCBI): 958
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.

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