Anticorps Recombinant de lapin anti-CD40L/CD154

CD40L/CD154 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240743F5

N° de cat : 83884-3-RR

Synonymes

CD154, Cd40l, 240743F5, CD40 ligand, Cd40lg

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Galerie de données de validation

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  WB analysis using 83884-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83884-3-RR (CD40L/CD154 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83884-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83884-3-RR against Mouse CD40L/CD154 were performed. The affinity constant is 0.406 nM.

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Applications testées

• Résultats positifs en WB: J774A.1 cells, CTLL-2 cells

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:18000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83884-3-RR cible CD40L/CD154 dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD40L/CD154 Protéine recombinante Eg0990
• Nom complet: CD40 ligand
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: NM_011616.2
• Symbole du gène: CD154
• Identification du gène (NCBI): 21947
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or an 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.

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