Anticorps Recombinant de lapin anti-CD40

CD40 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241760A4

N° de cat : 84406-1-PBS

Synonymes

241760A4, B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor

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Galerie de données de validation

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  WB analysis using 84406-1-RR (same clone as 84406-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84406-1-RR (CD40/TNFRSF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84406-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84406-1-RR (same clone as 84406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84406-1-RR (CD40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84406-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84406-1-RR (same clone as 84406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84406-1-RR (CD40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84406-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84406-1-PBS cible CD40 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD40 Protéine recombinante Eg1797
• Nom complet: CD40 molecule, TNF receptor superfamily member 5
• Masse moléculaire calculée: 31kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: NM_001250.6
• Symbole du gène: CD40
• Identification du gène (NCBI): 958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.

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