• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anticorps Recombinant de lapin anti-CD40

CD40 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241760A11

N° de cat : 84406-2-PBS

Synonymes

241760A11, B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using CD40 Recombinant antibody (84406-2-RR)

    WB analysis using 84406-2-RR (same clone as 84406-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84406-2-RR (CD40/TNFRSF5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84406-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD40 Recombinant antibody (84406-2-RR)

    IF Staining of Raji using 84406-2-RR (same clone as 84406-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD40/TNFRSF5 antibody (84406-2-RR, Clone: 241760A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84406-2-PBS in a different storage buffer formulation.

Informations sur le produit

84406-2-PBS cible CD40 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène CD40 Protéine recombinante Eg1797
Nom complet CD40 molecule, TNF receptor superfamily member 5
Masse moléculaire calculée31kDa
Poids moléculaire observé45 kDa
Numéro d’acquisition GenBankNM_001250.6
Symbole du gène CD40
Identification du gène (NCBI) 958
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.



{{ptg:RelatedPrimaryAntibodies}}