Anticorps Recombinant de lapin anti-CD40L/CD154

CD40L/CD154 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240743E6

N° de cat : 98120-1-RR

Synonymes

CD154, Cd40l, Tnfsf5, Cd40lg, CD40 ligand

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Galerie de données de validation

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  FC experiment of mouse CD3+ T cells using 98120-1-RR

  1x10^6 untreated or PMA and ionomycin treated mouse CD3+ T cells were surface stained with 0.25 ug Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody (98120-1-RR, Clone: 240743E6) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed.

• 

  FC experiment of mouse CD3+ T cells using 98120-1-RR

  1x10^6 PMA and ionomycin treated mouse CD3+ T cells were surface stained with 0.25 ug Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody (98120-1-RR, Clone: 240743E6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated mouse CD3+ T cells were surface stained with 0.25 ug Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody (98120-1-RR, Clone: 240743E6) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98120-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98120-1-RR against Mouse CD40L/CD154 were performed. The affinity constant is 0.989 nM.

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Applications testées

• Résultats positifs en cytométrie: PMA and ionomycin treated mouse CD3+ T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98120-1-RR cible CD40L/CD154 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD40 ligand
• Masse moléculaire calculée: 29 kDa
• Numéro d’acquisition GenBank: NM_011616.2
• Symbole du gène: CD154
• Identification du gène (NCBI): 21947
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.

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