Anticorps Recombinant de lapin anti-CD40L/CD154

CD40L/CD154 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242847F7

N° de cat : 98355-2-PBS

Synonymes

CD154, CD40L; CD154, CD40LG, CD 154, CD 40L

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using 98355-2-RR (same clone as 98355-2-PBS)

  1x10^6 untreated or PMA and ionomycin-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone: 242847F7) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD4. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98355-2-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98355-2-RR (same clone as 98355-2-PBS)

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone:242847F7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone: 242847F7) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98355-2-PBS in a different storage buffer formulation.

---

Informations sur le produit

98355-2-PBS cible CD40L/CD154 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD40 ligand
• Masse moléculaire calculée: 261 aa, 29 kDa
• Numéro d’acquisition GenBank: BC071754
• Symbole du gène: CD40 Ligand
• Identification du gène (NCBI): 959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.

{{ptg:RelatedPrimaryAntibodies}}