Anticorps Recombinant de lapin anti-CD40L/CD154

CD40L/CD154 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242847F7

N° de cat : 98355-2-RR

Synonymes

CD154, CD40L; CD154, CD40LG, CD 154, CD 40L

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Galerie de données de validation

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  FC experiment of human PBMCs using 98355-2-RR

  1x10^6 untreated or PMA and ionomycin-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone: 242847F7) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD4. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98355-2-RR

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone:242847F7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone: 242847F7) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: PMA and ionomycin treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98355-2-RR cible CD40L/CD154 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD40 ligand
• Masse moléculaire calculée: 261 aa, 29 kDa
• Numéro d’acquisition GenBank: BC071754
• Symbole du gène: CD40 Ligand
• Identification du gène (NCBI): 959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.

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