Anticorps Recombinant de lapin anti-CD40L/CD154

CD40L/CD154 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

240743E6

N° de cat : APC-FcA98120

Synonymes

CD154, Cd40l, CD40 ligand, Cd40lg, Tnfsf5

---

Galerie de données de validation

• 

  FC experiment of mouse CD3+ T cells using APC-FcA98120

  1x10^6 untreated or PMA and ionomycin treated mouse CD3+ T cells were surface stained with PE Anti-Mouse CD4 (RM4-4) and 0.1 ug APC Anti-Mouse CD40L/CD154 Rabbit RecAb (APC-FcA98120, Clone: 240743E6). Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: PMA and ionomycin treated mouse CD3+ T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-FcA98120 cible CD40L/CD154 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD40L/CD154 Protéine recombinante Eg0990
• Nom complet: CD40 ligand
• Masse moléculaire calculée: 29 kDa
• Numéro d’acquisition GenBank: NM_011616.2
• Symbole du gène: CD154
• Identification du gène (NCBI): 21947
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.

{{ptg:RelatedPrimaryAntibodies}}