Anticorps Recombinant de lapin anti-CD40L/CD154

CD40L/CD154 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

242847F7

N° de cat : CL750-98355-2

Synonymes

CD154, CD40L; CD154, CD40LG, CD 154, CD 40L

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Galerie de données de validation

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  FC experiment of human PBMCs using CL750-98355-2

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD4 and 5 ul CoraLite® Plus 750 Anti-Human CD40L/CD154 Rabbit RecAb (CL750-98355-2, Clone: 242847F7). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL750-98355-2

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD40L/CD154 Rabbit RecAb (CL750-98355-2, Clone: 242847F7) (red) or CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD40L/CD154 Rabbit RecAb (CL750-98355-2, Clone: 242847F7) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: PMA and ionomycin treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-98355-2 cible CD40L/CD154 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD40L/CD154 Protéine recombinante Eg3501
• Nom complet: CD40 ligand
• Masse moléculaire calculée: 261 aa, 29 kDa
• Numéro d’acquisition GenBank: BC071754
• Symbole du gène: CD40 Ligand
• Identification du gène (NCBI): 959
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.

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