Anticorps Monoclonal anti-CD41

CD41 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain, primates non humains

Applications

FC

Conjugaison

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

HIP8

N° de cat : CL405-65173

Synonymes

CD41a, CD41B, GP2B, GPalpha IIb, GPIIb

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Galerie de données de validation

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  FC experiment of human peripheral blood platelets using CL405-65173

  100 ul human platelet-rich plasma (diluted 1:2 with PBS) was surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD41a (CL405-65173, Clone:HIP8) or CoraLite® Plus 405 Mouse IgG1 Isotype Control (CL405-65124). Cells were not fixed.

• 

  FC experiment of human peripheral blood platelets using CL405-65173

  100 ul human platelet-rich plasma (diluted 1:2 with PBS) was surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD41a (CL405-65173, Clone:HIP8) or CoraLite® Plus 405 Mouse IgG1 Isotype Control (CL405-65124). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: plaquettes de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL405-65173 cible CD41 dans les applications de FC et montre une réactivité avec des échantillons Humain, primates non humains

• Réactivité: Humain, primates non humains
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• Numéro d’acquisition GenBank: BC117443
• Symbole du gène: CD41
• Identification du gène (NCBI): 3674
• Conjugaison: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Forme: Liquide
• Méthode de purification: Unknow
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID: 19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light beta (25 kDa) and heavy alpha (125 kDa) chains, which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. CD41/CD61 is crucial for platelet aggregation through binding of soluble fibrinogen. The HIP8 clone antibody specifically binds to the alpha chain of CD41.

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